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My account. ITEM : Alert me if price drops Full Name:. Email Address:. Phone Number:. Alert me if price drops below:. ZIP Code:. ITEM : HiLoad Superdex 75 pg prepacked columns are for high-resolution size exclusion chromatography of recombinant proteins from samples up to 5 mL or 13 mL.
Gel filtration column selectivity This column is packed with Superdex 75 prep grade pg resin. This SEC resin combines excellent mechanical strength with high hydrophilicity, allowing high flow rates and minimal nonspecific interactions.
Successful use of Superdex 75 columns in research These size exclusion columns are well suited to many applications, including: Purification of helicase RHAU proteins to characterize G-quaduplex recognition Purification of bacteriophage proteinsfor crystallization to study Cas3 inhibition Isolation of botulinum toxin proteins to study breast cancer cell activation Other size exclusion chromatography columns offers a variety of Life Sciences solutions for gel filtration size exclusion chromatography.
Among these are columns for analysis, small-scale purification, or separation across a broader molecular weight range. Click to close full size. Technical Specs. For more information on the batch specific expiration date, please contact technical service. There are no questions about this product yet. Be the first to ask a question. Add Question. Review this product Tell us about your personal experience Write a review.
Only buyers of this product can post a review. Are you sure you want to report this content? No Yes. HiLoad columns: Superdex 30 prep grade , Superdex 75 prep.
All are prep acked to. Fig 1. HiLoad Superdex 30, 75, and prep grade columns bring. Superdex 75 prep grade , and 10 to for. Fig 3. Fig 2. Selectivity curves from Superdex 30 pg, Superdex 75 pg, and. Fig 4. Comparison of the selectivity of Superdex 75 prep grade and. Superdex prep grade for model proteins. Superdex 75 prep grade A gives. The ferritin 5 contains. Table 1. Characteristics of HiLoad Superdex 30 prep grade ,. Figure 5. Superdex 30 prep grade , Superdex 75 prep grade , and.
Superdex prep grade may be used in aqueous. Fig 5. Performance of Superdex prep grade measured as K av. The XK columns prep acked with Superdex prep grade media. HiLoad Superdex 30 prep grade is optimized for proteins. HiLoad Superdex 75 prep grade separates proteins and.
Fig 6. The numbers above each peak indicate the number. Fig 9. Two ml concentrated sample of the endoglucanase. HiLoad Superdex prep grade has a separation range of. Fig Superdex 30 prep grade ml Superdex 75 prep grade ml Superdex prep grade ml All goods and services are sold subject to the terms and conditions of sale of the company within. GE Healthcare which supplies them.
A copy of these terms and conditions is available on request. Each column is expertly packed and individually tested. Extended embed settings. You have already flagged this document.
Thank you, for helping us keep this platform clean. The editors will have a look at it as soon as possible. Self publishing. Share Embed Flag. TAGS superdex prep hiload column columns healthcare prepacked volume separation proteins www. Superdex prep grade is a matrix of dextran and highly cross-linked agarose. All are prep acked to mm bed heights in 16 mm or 26 mm diameter columns. This combination of prep acked convenience and reproducibility make HiLoad Superdex prep grade columns a confident choice for fast, high-resolution gel filtration at prep arative scale.
Superdex gel filtration media are produced by the covalent binding of dextran to highly cross-linked porous agarose beads. HiLoad Superdex 30, 75, and prep grade columns bring convenience and high resolution to gel filtration. Steep selectivity curves give unmatched resolution for biomolecules in the molecular weight range up to 10 for Superdex 30 prep grade Fig 1 , to 70 for Superdex 75 prep grade , and 10 to for Superdex prep grade Fig 2.
Myoglobulin, 1. IgG, 0. Ferritin, 0. Selectivity curves from Superdex 30 pg, Superdex 75 pg, and Superdex pg. Table 1 summarizes the characteristics of the media and columns. Comparison of the selectivity of Superdex 75 prep grade and Superdex prep grade for model proteins. Superdex 75 prep grade A gives excellent resolution of the three proteins in the M r range 17 to 67 while the two largest elute together in the void volume.
Superdex prep grade B resolves these two largest proteins. The ferritin 5 contains aggregates and thus results in a double peak. All strong oxidizing agents should, however, be avoided.
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